Acute hypoxia induces upregulation of microRNA-210 expression in glioblastoma spheroids

Tine Agerbo  Rosenberg; Mads Thomassen; Stine Skov Jensen; Martin Jakob Larsen; Kristina Pilekær  Sørensen; Simon Kjær Hermansen; Torben A Kruse; Bjarne Winther Kristensen

doi:10.2217/cns.14.48

Acute hypoxia induces upregulation of microRNA-210 expression in glioblastoma spheroids

Tine Agerbo Rosenberg, Mads Thomassen, Stine Skov Jensen, Martin Jakob Larsen, Kristina Pilekær Sørensen, Simon Kjær Hermansen, Torben A Kruse, Bjarne Winther Kristensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

AIM: Tumor hypoxia and presence of tumor stem cells are related to therapeutic resistance and tumorigenicity in glioblastomas. The aim of the present study was therefore to identify microRNAs deregulated in acute hypoxia and to identify possible associated changes in stem cell markers.

MATERIALS & METHODS: Glioblastoma spheroid cultures were grown in either 2 or 21% oxygen. Subsequently, miRNA profiling was performed and expression of ten stem cell markers was examined.

RESULTS: MiRNA-210 was significantly upregulated in hypoxia in patient-derived spheroids. The stem cell markers displayed a complex regulatory pattern.

CONCLUSION: MiRNA-210 appears to be upregulated in hypoxia in immature glioblastoma cells. This miRNA may represent a therapeutic target although it is not clear from the results whether this miRNA may be related to specific cancer stem cell functions.

Originalsprog	Engelsk
Tidsskrift	CNS Oncology
Vol/bind	4
Udgave nummer	1
Sider (fra-til)	25-35
Antal sider	11
ISSN	2045-0907
DOI	https://doi.org/10.2217/cns.14.48
Status	Udgivet - 2015

Dokumenter og links

10.2217/cns.14.48

http://www.futuremedicine.com/doi/abs/10.2217/cns.14.48

Citationsformater

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abstract = "AIM: Tumor hypoxia and presence of tumor stem cells are related to therapeutic resistance and tumorigenicity in glioblastomas. The aim of the present study was therefore to identify microRNAs deregulated in acute hypoxia and to identify possible associated changes in stem cell markers.MATERIALS & METHODS: Glioblastoma spheroid cultures were grown in either 2 or 21% oxygen. Subsequently, miRNA profiling was performed and expression of ten stem cell markers was examined.RESULTS: MiRNA-210 was significantly upregulated in hypoxia in patient-derived spheroids. The stem cell markers displayed a complex regulatory pattern.CONCLUSION: MiRNA-210 appears to be upregulated in hypoxia in immature glioblastoma cells. This miRNA may represent a therapeutic target although it is not clear from the results whether this miRNA may be related to specific cancer stem cell functions.",

author = "Rosenberg, {Tine Agerbo} and Mads Thomassen and Jensen, {Stine Skov} and Larsen, {Martin Jakob} and S{\o}rensen, {Kristina Pilek{\ae}r} and Hermansen, {Simon Kj{\ae}r} and Kruse, {Torben A} and Kristensen, {Bjarne Winther}",

year = "2015",

doi = "10.2217/cns.14.48",

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volume = "4",

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T1 - Acute hypoxia induces upregulation of microRNA-210 expression in glioblastoma spheroids

AU - Rosenberg, Tine Agerbo

AU - Thomassen, Mads

AU - Jensen, Stine Skov

AU - Larsen, Martin Jakob

AU - Sørensen, Kristina Pilekær

AU - Hermansen, Simon Kjær

AU - Kruse, Torben A

AU - Kristensen, Bjarne Winther

PY - 2015

Y1 - 2015

N2 - AIM: Tumor hypoxia and presence of tumor stem cells are related to therapeutic resistance and tumorigenicity in glioblastomas. The aim of the present study was therefore to identify microRNAs deregulated in acute hypoxia and to identify possible associated changes in stem cell markers.MATERIALS & METHODS: Glioblastoma spheroid cultures were grown in either 2 or 21% oxygen. Subsequently, miRNA profiling was performed and expression of ten stem cell markers was examined.RESULTS: MiRNA-210 was significantly upregulated in hypoxia in patient-derived spheroids. The stem cell markers displayed a complex regulatory pattern.CONCLUSION: MiRNA-210 appears to be upregulated in hypoxia in immature glioblastoma cells. This miRNA may represent a therapeutic target although it is not clear from the results whether this miRNA may be related to specific cancer stem cell functions.

AB - AIM: Tumor hypoxia and presence of tumor stem cells are related to therapeutic resistance and tumorigenicity in glioblastomas. The aim of the present study was therefore to identify microRNAs deregulated in acute hypoxia and to identify possible associated changes in stem cell markers.MATERIALS & METHODS: Glioblastoma spheroid cultures were grown in either 2 or 21% oxygen. Subsequently, miRNA profiling was performed and expression of ten stem cell markers was examined.RESULTS: MiRNA-210 was significantly upregulated in hypoxia in patient-derived spheroids. The stem cell markers displayed a complex regulatory pattern.CONCLUSION: MiRNA-210 appears to be upregulated in hypoxia in immature glioblastoma cells. This miRNA may represent a therapeutic target although it is not clear from the results whether this miRNA may be related to specific cancer stem cell functions.

U2 - 10.2217/cns.14.48

DO - 10.2217/cns.14.48

M3 - Journal article

C2 - 25586423

SN - 2045-0907

VL - 4

SP - 25

EP - 35

JO - CNS Oncology

JF - CNS Oncology

IS - 1

ER -

Acute hypoxia induces upregulation of microRNA-210 expression in glioblastoma spheroids

Abstract

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