Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Dominik Kopczynski; Andreas Hentschel; Cristina Coman; Nils Helge Schebb; Thorsten Hornemann; Douglas G. Mashek; Nicole M. Hartung; Olga Shevchuk; Hans Frieder Schött; Kristina Lorenz; Federico Torta; Bo Burla; René P. Zahedi; Albert Sickmann; Michael R. Kreutz; Christer S. Ejsing; Jan Medenbach; Robert Ahrends

doi:10.1021/acs.analchem.0c02793

Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Dominik Kopczynski, Andreas Hentschel, Cristina Coman, Nils Helge Schebb, Thorsten Hornemann, Douglas G. Mashek, Nicole M. Hartung, Olga Shevchuk, Hans Frieder Schött, Kristina Lorenz, Federico Torta, Bo Burla, René P. Zahedi, Albert Sickmann, Michael R. Kreutz, Christer S. Ejsing, Jan Medenbach, Robert Ahrends^*

^*Kontaktforfatter

Institut for Biokemi og Molekylær Biologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

Originalsprog	Engelsk
Tidsskrift	Analytical Chemistry
Vol/bind	92
Udgave nummer	20
Sider (fra-til)	13672-13676
ISSN	0003-2700
DOI	https://doi.org/10.1021/acs.analchem.0c02793
Status	Udgivet - 20. okt. 2020

Dokumenter og links

10.1021/acs.analchem.0c02793Licens: CC BY

Open Access VersionForlagets udgivne version, 2,05 MBLicens: CC BY

Citationsformater

Kopczynski, D., Hentschel, A., Coman, C., Schebb, N. H., Hornemann, T., Mashek, D. G., Hartung, N. M., Shevchuk, O., Schött, H. F., Lorenz, K., Torta, F., Burla, B., Zahedi, R. P., Sickmann, A., Kreutz, M. R., Ejsing, C. S., Medenbach, J., & Ahrends, R. (2020). Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics. Analytical Chemistry, 92(20), 13672-13676. https://doi.org/10.1021/acs.analchem.0c02793

@article{950e050d9b9f4495984a823149a4a2f7,

title = "Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics",

abstract = "We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms. ",

author = "Dominik Kopczynski and Andreas Hentschel and Cristina Coman and Schebb, {Nils Helge} and Thorsten Hornemann and Mashek, {Douglas G.} and Hartung, {Nicole M.} and Olga Shevchuk and Sch{\"o}tt, {Hans Frieder} and Kristina Lorenz and Federico Torta and Bo Burla and Zahedi, {Ren{\'e} P.} and Albert Sickmann and Kreutz, {Michael R.} and Ejsing, {Christer S.} and Jan Medenbach and Robert Ahrends",

year = "2020",

month = oct,

day = "20",

doi = "10.1021/acs.analchem.0c02793",

language = "English",

volume = "92",

pages = "13672--13676",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "20",

}

Kopczynski, D, Hentschel, A, Coman, C, Schebb, NH, Hornemann, T, Mashek, DG, Hartung, NM, Shevchuk, O, Schött, HF, Lorenz, K, Torta, F, Burla, B, Zahedi, RP, Sickmann, A, Kreutz, MR, Ejsing, CS, Medenbach, J & Ahrends, R 2020, 'Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics', Analytical Chemistry, bind 92, nr. 20, s. 13672-13676. https://doi.org/10.1021/acs.analchem.0c02793

TY - JOUR

T1 - Simple Targeted Assays for Metabolic Pathways and Signaling

T2 - A Powerful Tool for Targeted Proteomics

AU - Kopczynski, Dominik

AU - Hentschel, Andreas

AU - Coman, Cristina

AU - Schebb, Nils Helge

AU - Hornemann, Thorsten

AU - Mashek, Douglas G.

AU - Hartung, Nicole M.

AU - Shevchuk, Olga

AU - Schött, Hans Frieder

AU - Lorenz, Kristina

AU - Torta, Federico

AU - Burla, Bo

AU - Zahedi, René P.

AU - Sickmann, Albert

AU - Kreutz, Michael R.

AU - Ejsing, Christer S.

AU - Medenbach, Jan

AU - Ahrends, Robert

PY - 2020/10/20

Y1 - 2020/10/20

N2 - We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

AB - We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

U2 - 10.1021/acs.analchem.0c02793

DO - 10.1021/acs.analchem.0c02793

M3 - Journal article

C2 - 32865986

AN - SCOPUS:85096651938

SN - 0003-2700

VL - 92

SP - 13672

EP - 13676

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 20

ER -

Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Abstract

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