TY - JOUR
T1 - Renal cortical and medullary blood flow responses to altered NO-availability in humans
AU - Damkjaer, Mads
AU - Vafaee, Manoucher
AU - Møller, Michael Lehd
AU - Braad, Poul Erik
AU - Petersen, Henrik
AU - Høilund-Carlsen, Poul Flemming
AU - Bie, Peter
PY - 2010/9/29
Y1 - 2010/9/29
N2 - The objective was to quantify regional renal blood flow in humans. In nine young volunteers on a controlled diet, the lower abdomen was CT-scanned and regional renal blood flow determined by positron emission tomography (PET) scanning using H(2)(15)O as tracer. Measurements were performed at baseline, during constant intravenous infusion of nitric oxide (NO) donor (glyceryl nitrate), and after intravenous injection of NO synthase inhibitor (L-NMMA). Using the CT-image, the kidney pole areas were delineated as volumes of interest (VOI). In the data analysis, tissue layers with a thickness of one voxel were eliminated stepwise from the external surface of the VOI ('voxel peeling'), and the blood flow subsequently determined in each new, reduced VOI. Blood flow in the shrinking volumes of interest (VOIs) decreased as the number of cycles of voxel peeling increased. After 4-5 cycles, blood flow was not reduced further by additional voxel peeling. This volume-insensitive flow was measured to be 2.30 ±0.17 ml·(g·min)(-1) during the control period; it increased during infusion of glyceryl nitrate to 2.97 ±0.18 ml·(g·min)(-1) (p
AB - The objective was to quantify regional renal blood flow in humans. In nine young volunteers on a controlled diet, the lower abdomen was CT-scanned and regional renal blood flow determined by positron emission tomography (PET) scanning using H(2)(15)O as tracer. Measurements were performed at baseline, during constant intravenous infusion of nitric oxide (NO) donor (glyceryl nitrate), and after intravenous injection of NO synthase inhibitor (L-NMMA). Using the CT-image, the kidney pole areas were delineated as volumes of interest (VOI). In the data analysis, tissue layers with a thickness of one voxel were eliminated stepwise from the external surface of the VOI ('voxel peeling'), and the blood flow subsequently determined in each new, reduced VOI. Blood flow in the shrinking volumes of interest (VOIs) decreased as the number of cycles of voxel peeling increased. After 4-5 cycles, blood flow was not reduced further by additional voxel peeling. This volume-insensitive flow was measured to be 2.30 ±0.17 ml·(g·min)(-1) during the control period; it increased during infusion of glyceryl nitrate to 2.97 ±0.18 ml·(g·min)(-1) (p
U2 - 10.1152/ajpregu.00440.2010
DO - 10.1152/ajpregu.00440.2010
M3 - Journal article
C2 - 20881099
SN - 0363-6119
VL - 299
SP - 1449
EP - 1455
JO - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology
JF - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology
IS - 6
ER -