Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin

Nicole L. Henriksen; Karoline Aasmul-Olsen; Ramakrishnan Venkatasubramanian; Mikkel K.E. Nygaard; Richard R. Sprenger; Anne B. Heckmann; Marie S. Ostenfeld; Christer S. Ejsing; Simon F. Eskildsen; Anette Müllertz; Per T. Sangild; Stine B. Bering; Thomas Thymann

doi:10.3390/nu13030718

Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin

Nicole L. Henriksen, Karoline Aasmul-Olsen, Ramakrishnan Venkatasubramanian, Mikkel K.E. Nygaard, Richard R. Sprenger, Anne B. Heckmann, Marie S. Ostenfeld, Christer S. Ejsing, Simon F. Eskildsen, Anette Müllertz, Per T. Sangild, Stine B. Bering, Thomas Thymann^*

^*Kontaktforfatter

Institut for Biokemi og Molekylær Biologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

Breastfed infants have higher intestinal lipid absorption and neurodevelopmental outcomes compared to formula-fed infants, which may relate to a different surface layer structure of fat globules in infant formula. This study investigated if dairy-derived emulsifiers increased lipid absorption and neurodevelopment relative to soy lecithin in newborn preterm piglets. Piglets received a formula diet containing soy lecithin (SL) or whey protein concentrate enriched in extracellular vesicles (WPC-A-EV) or phospholipids (WPC-PL) for 19 days. Both WPC-A-EV and WPC-PL emulsions, but not the intact diets, increased in vitro lipolysis compared to SL. The main differences of plasma lipidomics analysis were increased levels of some sphingolipids, and lipid molecules with odd-chain (17:1, 19:1, 19:3) as well as mono-and polyunsaturated fatty acyl chains (16:1, 20:1, 20:3) in the WPC-A-EV and WPC-PL groups and increased 18:2 fatty acyls in the SL group. Indirect monitoring of intestinal triacylglycerol absorption showed no differences between groups. Diffusor tensor imaging measurements of mean diffusivity in the hippocampus were lower for WPC-A-EV and WPC-PL groups compared to SL indicating improved hippocampal maturation. No differences in hippocampal lipid composition or short-term memory were observed between groups. In conclu-sion, emulsification of fat globules in infant formula with dairy-derived emulsifiers altered the plasma lipid profile and hippocampal tissue diffusivity but had limited effects on other absorptive and learning abilities relative to SL in preterm piglets.

Originalsprog	Engelsk
Artikelnummer	718
Tidsskrift	Nutrients
Vol/bind	13
Udgave nummer	3
Antal sider	17
ISSN	2072-6643
DOI	https://doi.org/10.3390/nu13030718
Status	Udgivet - 2021

Bibliografisk note

Funding Information:
Funding: This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark.

Funding Information:
This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark. The authors would like to thank Britta Karlsson, Elin Skytte, Jane Povlsen, Ben-jamin Lambert-Z?berlein and Mette Ernst for their assistance in experimental animal work. Moreo-ver, we thank Kenneth Olsson, Ann-Dorit Moltke S?rensen, Charlotte Jacobsen and Lis Berner for their assistance in producing the emulsions. We also thank Yuki Mori and the Panum Preclinical MRI Core Facility for conducting the MRI scans. The late Lars Ingvar Hellgren, Technical University of Denmark, is acknowledged for his passionate initiation and steering of the InfantBrain project until his health condition prevented further involvement.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Dokumenter og links

10.3390/nu13030718Licens: CC BY

Open Access VersionForlagets udgivne version, 2,95 MBLicens: CC BY

Citationsformater

Henriksen, N. L., Aasmul-Olsen, K., Venkatasubramanian, R., Nygaard, M. K. E., Sprenger, R. R., Heckmann, A. B., Ostenfeld, M. S., Ejsing, C. S., Eskildsen, S. F., Müllertz, A., Sangild, P. T., Bering, S. B., & Thymann, T. (2021). Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin. Nutrients, 13(3), Artikel 718. https://doi.org/10.3390/nu13030718

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title = "Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin",

abstract = "Breastfed infants have higher intestinal lipid absorption and neurodevelopmental outcomes compared to formula-fed infants, which may relate to a different surface layer structure of fat globules in infant formula. This study investigated if dairy-derived emulsifiers increased lipid absorption and neurodevelopment relative to soy lecithin in newborn preterm piglets. Piglets received a formula diet containing soy lecithin (SL) or whey protein concentrate enriched in extracellular vesicles (WPC-A-EV) or phospholipids (WPC-PL) for 19 days. Both WPC-A-EV and WPC-PL emulsions, but not the intact diets, increased in vitro lipolysis compared to SL. The main differences of plasma lipidomics analysis were increased levels of some sphingolipids, and lipid molecules with odd-chain (17:1, 19:1, 19:3) as well as mono-and polyunsaturated fatty acyl chains (16:1, 20:1, 20:3) in the WPC-A-EV and WPC-PL groups and increased 18:2 fatty acyls in the SL group. Indirect monitoring of intestinal triacylglycerol absorption showed no differences between groups. Diffusor tensor imaging measurements of mean diffusivity in the hippocampus were lower for WPC-A-EV and WPC-PL groups compared to SL indicating improved hippocampal maturation. No differences in hippocampal lipid composition or short-term memory were observed between groups. In conclu-sion, emulsification of fat globules in infant formula with dairy-derived emulsifiers altered the plasma lipid profile and hippocampal tissue diffusivity but had limited effects on other absorptive and learning abilities relative to SL in preterm piglets.",

keywords = "Bovine whey phospholipids, Brain, Emulsification, Extracellular vesicles, Infant formula, Milk fat globule membrane, Neurodevelopment, Piglet, Polar lipids, Preterm",

author = "Henriksen, {Nicole L.} and Karoline Aasmul-Olsen and Ramakrishnan Venkatasubramanian and Nygaard, {Mikkel K.E.} and Sprenger, {Richard R.} and Heckmann, {Anne B.} and Ostenfeld, {Marie S.} and Ejsing, {Christer S.} and Eskildsen, {Simon F.} and Anette M{\"u}llertz and Sangild, {Per T.} and Bering, {Stine B.} and Thomas Thymann",

note = "Funding Information: Funding: This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark. Funding Information: This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark. The authors would like to thank Britta Karlsson, Elin Skytte, Jane Povlsen, Ben-jamin Lambert-Z?berlein and Mette Ernst for their assistance in experimental animal work. Moreo-ver, we thank Kenneth Olsson, Ann-Dorit Moltke S?rensen, Charlotte Jacobsen and Lis Berner for their assistance in producing the emulsions. We also thank Yuki Mori and the Panum Preclinical MRI Core Facility for conducting the MRI scans. The late Lars Ingvar Hellgren, Technical University of Denmark, is acknowledged for his passionate initiation and steering of the InfantBrain project until his health condition prevented further involvement. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

doi = "10.3390/nu13030718",

language = "English",

volume = "13",

journal = "Nutrients",

issn = "2072-6643",

publisher = "MDPI",

number = "3",

}

Henriksen, NL, Aasmul-Olsen, K, Venkatasubramanian, R, Nygaard, MKE, Sprenger, RR, Heckmann, AB, Ostenfeld, MS, Ejsing, CS, Eskildsen, SF, Müllertz, A, Sangild, PT, Bering, SB & Thymann, T 2021, 'Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin', Nutrients, bind 13, nr. 3, 718. https://doi.org/10.3390/nu13030718

Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin. / Henriksen, Nicole L.; Aasmul-Olsen, Karoline; Venkatasubramanian, Ramakrishnan et al.
I: Nutrients, Bind 13, Nr. 3, 718, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin

AU - Henriksen, Nicole L.

AU - Aasmul-Olsen, Karoline

AU - Venkatasubramanian, Ramakrishnan

AU - Nygaard, Mikkel K.E.

AU - Sprenger, Richard R.

AU - Heckmann, Anne B.

AU - Ostenfeld, Marie S.

AU - Ejsing, Christer S.

AU - Eskildsen, Simon F.

AU - Müllertz, Anette

AU - Sangild, Per T.

AU - Bering, Stine B.

AU - Thymann, Thomas

N1 - Funding Information: Funding: This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark. Funding Information: This research was funded by the Innovation Fund Denmark (Grant no.: 5158-00014B), the Dairy Research Foundation and Arla Foods Ingredients Denmark. The authors would like to thank Britta Karlsson, Elin Skytte, Jane Povlsen, Ben-jamin Lambert-Z?berlein and Mette Ernst for their assistance in experimental animal work. Moreo-ver, we thank Kenneth Olsson, Ann-Dorit Moltke S?rensen, Charlotte Jacobsen and Lis Berner for their assistance in producing the emulsions. We also thank Yuki Mori and the Panum Preclinical MRI Core Facility for conducting the MRI scans. The late Lars Ingvar Hellgren, Technical University of Denmark, is acknowledged for his passionate initiation and steering of the InfantBrain project until his health condition prevented further involvement. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021

Y1 - 2021

N2 - Breastfed infants have higher intestinal lipid absorption and neurodevelopmental outcomes compared to formula-fed infants, which may relate to a different surface layer structure of fat globules in infant formula. This study investigated if dairy-derived emulsifiers increased lipid absorption and neurodevelopment relative to soy lecithin in newborn preterm piglets. Piglets received a formula diet containing soy lecithin (SL) or whey protein concentrate enriched in extracellular vesicles (WPC-A-EV) or phospholipids (WPC-PL) for 19 days. Both WPC-A-EV and WPC-PL emulsions, but not the intact diets, increased in vitro lipolysis compared to SL. The main differences of plasma lipidomics analysis were increased levels of some sphingolipids, and lipid molecules with odd-chain (17:1, 19:1, 19:3) as well as mono-and polyunsaturated fatty acyl chains (16:1, 20:1, 20:3) in the WPC-A-EV and WPC-PL groups and increased 18:2 fatty acyls in the SL group. Indirect monitoring of intestinal triacylglycerol absorption showed no differences between groups. Diffusor tensor imaging measurements of mean diffusivity in the hippocampus were lower for WPC-A-EV and WPC-PL groups compared to SL indicating improved hippocampal maturation. No differences in hippocampal lipid composition or short-term memory were observed between groups. In conclu-sion, emulsification of fat globules in infant formula with dairy-derived emulsifiers altered the plasma lipid profile and hippocampal tissue diffusivity but had limited effects on other absorptive and learning abilities relative to SL in preterm piglets.

AB - Breastfed infants have higher intestinal lipid absorption and neurodevelopmental outcomes compared to formula-fed infants, which may relate to a different surface layer structure of fat globules in infant formula. This study investigated if dairy-derived emulsifiers increased lipid absorption and neurodevelopment relative to soy lecithin in newborn preterm piglets. Piglets received a formula diet containing soy lecithin (SL) or whey protein concentrate enriched in extracellular vesicles (WPC-A-EV) or phospholipids (WPC-PL) for 19 days. Both WPC-A-EV and WPC-PL emulsions, but not the intact diets, increased in vitro lipolysis compared to SL. The main differences of plasma lipidomics analysis were increased levels of some sphingolipids, and lipid molecules with odd-chain (17:1, 19:1, 19:3) as well as mono-and polyunsaturated fatty acyl chains (16:1, 20:1, 20:3) in the WPC-A-EV and WPC-PL groups and increased 18:2 fatty acyls in the SL group. Indirect monitoring of intestinal triacylglycerol absorption showed no differences between groups. Diffusor tensor imaging measurements of mean diffusivity in the hippocampus were lower for WPC-A-EV and WPC-PL groups compared to SL indicating improved hippocampal maturation. No differences in hippocampal lipid composition or short-term memory were observed between groups. In conclu-sion, emulsification of fat globules in infant formula with dairy-derived emulsifiers altered the plasma lipid profile and hippocampal tissue diffusivity but had limited effects on other absorptive and learning abilities relative to SL in preterm piglets.

KW - Bovine whey phospholipids

KW - Brain

KW - Emulsification

KW - Extracellular vesicles

KW - Infant formula

KW - Milk fat globule membrane

KW - Neurodevelopment

KW - Piglet

KW - Polar lipids

KW - Preterm

U2 - 10.3390/nu13030718

DO - 10.3390/nu13030718

M3 - Journal article

C2 - 33668360

AN - SCOPUS:85101297893

SN - 2072-6643

VL - 13

JO - Nutrients

JF - Nutrients

IS - 3

M1 - 718

ER -

Dairy-derived emulsifiers in infant formula show marginal effects on the plasma lipid profile and brain structure in preterm piglets relative to soy lecithin

Abstract

Bibliografisk note

Dokumenter og links

Fingeraftryk

Citationsformater